What+is+a+Microarray?

A. **__Basic Microarray__** 1. Small, solid support onto which thousands of gene sequences attach or fix at a single location 2. Supports are usually glass microscope slides a. Generally only as big as two pinky fingers side by side b. Can also be silicon chips or nylon membranes 3. The DNA gets synthesized directly onto the support a. DNA b. cDNA c. **Oligonucleotide**- a short fragment of single stranded DNA, typically 5-50 C. There are three basic types of samples that can be used to construct DNA Microarrays. Two are genomic and the other is Transcriptomic. Transcriptomic- measures the mRNA levels

A Microarray is a sequence of dots of DNA, protein, and tissue arranged on a silicon, glass, or nylon membrane slide. DNA microarrays play an integral role in gene expression profiling. 1: Healthy Tissue Sample and Non-Healthy Tissue Samples are collected 2: Both Healthy and Non-healthy cell's mRNA is extracted 3: mRNA is changed into cDNA and tagged with flourescent probes. Healthy cell's cDNA is tagged green and non-Healthy cell's cDNA is tagged red. 4: Both healthy and non-healthy cDNA are added to the microarray. cDNA hybridizes with the probes on the microarray 5: Microarray is read using computer optics and is regenerated on a computer screen showing which cell's cDNA hybridized with which probes on the microarray 6: The final microarray is shown in color. Red for the non-healthy cell's cDNA. Green for the healthy cell's cDNA. orange-yellow for both healthy and non healthy. black for nothing.